Plasmid

Part:BBa_K3734017:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


GIP-miR21T-GI-GAL4-4XmiR21T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4624
    Illegal NotI site found at 1438
    Illegal NotI site found at 4997
    Illegal NotI site found at 5479
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal BglII site found at 4519
    Illegal XhoI site found at 2172
    Illegal XhoI site found at 5229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1127
    Illegal NgoMIV site found at 2674
    Illegal NgoMIV site found at 6055
    Illegal NgoMIV site found at 7397
    Illegal NgoMIV site found at 7680
    Illegal AgeI site found at 3901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5148
    Illegal BsaI.rc site found at 444
    Illegal BsaI.rc site found at 3298
    Illegal BsaI.rc site found at 9208
    Illegal SapI site found at 8125
    Illegal SapI.rc site found at 7246
    Illegal SapI.rc site found at 7456


Design Notes

We use 4XmiR21T at 3'UTR.When you use PCR to clone the fragment, you need to focus on how many times the miR21T repeats.


Source

GIP and miR21T come from human genome.GI is from Arabidopsis thaliana.GAL4 is from Saccharomyces cerevisiae.

References